Effect of glycerol concentration on the motility of equine spermatozoa after thawing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F17%3A43875703" target="_blank" >RIV/62157124:16170/17:43875703 - isvavai.cz</a>

Result on the web

<a href="https://actavet.vfu.cz/86/3/0263/" target="_blank" >https://actavet.vfu.cz/86/3/0263/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201786030263" target="_blank" >10.2754/avb201786030263</a>

Result language

angličtina

Original language name

Effect of glycerol concentration on the motility of equine spermatozoa after thawing

Original language description

The aim of this study was to evaluate the effect of different glycerol concentrations on stallion sperm motility after thawing. For statistical analysis 228 ejaculates were used. The semen was filtrated to remove gel fraction; macroscopic and microscopic evaluation was done. After evaluation the ejaculates were centrifuged, the supernatant was removed and the spermatozoa were re-suspended in French diluent with different concentrations of glycerol (2.0; 2.5; 4.0 and 6.0%). The choice of concentration of glycerol for a particular ejaculate was completely random. The spermatozoa were packed into 0.5 ml straws and placed for 2 h in a fridge (4 degrees C). Then the straws were placed in liquid nitrogen vapor (-80 to -100 degrees C) and after 10 min plunged into liquid nitrogen and stored at -196 degrees C for at least 48 h. The selected straws were individually thawed in a 38 degrees C water bath for 30 s prior to post-freezing analysis. Two progressive motilities using phase contrast microscopy (magnification x 400) were recorded: motility II immediately after thawing and motility III after 2 h incubation in a 38 degrees C water bath. The Spearmen/Kendall rang correlation test was selected to prove whether there is a correlation between the selected indices (glycerol concentration and motility II and motility III). Nonparametric multiple group analysis (Steel-Dwass test) was applied for finding the differences between groups. The Spearman/Kendall rang correlation proved a relationship between motility II and glycerol concentration. It can be stated that in this study the best glycerol concentration for freezing equine spermatozoa is with a concentration of 4.0% glycerol.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

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Volume of the periodical

86

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

263-268

UT code for WoS article

000416306200008

EID of the result in the Scopus database

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