Antibiotic resistance and virulence factors in mesophilic Aeromonas spp. from Czech carp fisheries

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F18%3A43876440" target="_blank" >RIV/62157124:16170/18:43876440 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/18:43876440 RIV/62157124:16810/18:43876440

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/jam.14075" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/jam.14075</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jam.14075" target="_blank" >10.1111/jam.14075</a>

Result language

angličtina

Original language name

Antibiotic resistance and virulence factors in mesophilic Aeromonas spp. from Czech carp fisheries

Original language description

Aims The aim of this study was to determine phenotypic and genotypic resistance, virulence and clonal relationship of aeromonads and related species isolated from Czech carp fisheries. Methods and Results Forty-nine isolates obtained from a total of 154 fish from three breeding facilities were species identified using matrix-assisted laser desorption/ionization time of flight and the sequencing of the rpoB housekeeping gene. Most Aeromonas isolates were identified as Aeromonas veronii (94%, n = 34). Susceptibility to six antibiotics (oxytetracycline, flumequine, florfenicol, sulphamethoxazole/trimethoprim, enrofloxacin and oxolinic acid) was tested using the disc diffusion method. The presence of resistance genes and virulence factors was verified by PCR and sequencing, and the clonal relationship was analysed using pulsed-field gel electrophoresis (PFGE). Phenotypic resistance to one or more antimicrobials was found in 32 isolates (65%, n = 49). Resistance to oxytetracycline was the most common (41%) and associated mainly with the presence of tet(E) gene, while the percentage of isolates resistant to florfenicol was low (2%). Isolates carried one to five of the tested virulence factors and showed high diversity of PFGE profiles. Conclusions Since the highest percentage of antimicrobial resistance in aeromonads was found for oxytetracycline and the lowest percentage for florfenicol, it is suggested that florfenicol could be an adequate treatment alternative in carp fisheries. Significance and Impact of the Study Increasing resistance of aeromonads to commonly used antimicrobials has become an emerging problem in fisheries. This study was conducted in relation to the practical needs to identify a suitable antibiotic as an alternative to oxytetracycline.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

—

Volume of the periodical

125

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

1702-1713

UT code for WoS article

000450333400011

EID of the result in the Scopus database

2-s2.0-85053664203