Selection and characterization of scFv antibody against nucleocapsid protein of Porcine reproductive and respiratory syndrome virus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F20%3A43878680" target="_blank" >RIV/62157124:16170/20:43878680 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/20:43878680
Result on the web
<a href="https://actavet.vfu.cz/media/pdf/actavet_2020089010039.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2020089010039.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb202089010039" target="_blank" >10.2754/avb202089010039</a>
Alternative languages
Result language
angličtina
Original language name
Selection and characterization of scFv antibody against nucleocapsid protein of Porcine reproductive and respiratory syndrome virus
Original language description
Porcine reproductive and respiratory syndrome virus (PRRSV) is a widespread infectious agent in pigs. Nucleocapsid (N) protein of PRRSV has been identified as the most immunodominant viral protein. The main goal of the work was the selection and characterization of a single-chain antibody fragments (scFv) antibody specific to the N protein. Specific scFv antibody clone D5 was selected from the Tomlinson phagemid library and purified by immobilized metal affinity chromatography from the periplasmatic space of E. coli cells. The antibody was then characterized by sequencing and the ability to recognize the native virus N protein by Western blot and competitive ELISA. Pepscan analysis identified the position of the binding epitope between amino acids 62-84 of the N protein. Our study could help to improve the diagnostics and prevention of PRRSV in Central Europe.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta veterinaria Brno
ISSN
0001-7213
e-ISSN
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Volume of the periodical
89
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
39-45
UT code for WoS article
000529064300005
EID of the result in the Scopus database
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