Caspase-1 inhibition affects Cd36 expression and Rankl/Opg ratio in differentiating chondrocytes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F21%3A43879384" target="_blank" >RIV/62157124:16170/21:43879384 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Caspase-1 inhibition affects Cd36 expression and Rankl/Opg ratio in differentiating chondrocytes
Original language description
The conference publication follows the modulation of the expression of the fatty acid translocase Cd36 by caspase-1. This caspase plays role in inflammation, but has been studied also in relation to lipid metabolism, the same as Cd36. For this reason, connections between the two molecules were sought using chondrogenic micromass cultures treated by selective caspase-1 inhibitor. In the treated samples, the gene expression of Cd36 was significantly upregulated. Also, the expression of two important bone remodelling and cartilage/bone homeostasis markers was changed: The Opg gene was upregulated, whereas Rankl was downregulated. Immunocytofluorescence confirmed these modulations on protein level. Cd36 silencing caused opposing trends in Rankl and Opg expression. The presented results demonstrate novel functions of caspase-1 in chondrocyte differentiation and pathways connected with lipid metabolism through Cd36. The detected effect of Cd36 on the Rankl/Opg ratio is important in relation to bone remodelling and metabolism as well as pathologies such as osteoarthritis.
Czech name
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Czech description
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Project
<a href="/en/project/GA19-12023S" target="_blank" >GA19-12023S: Novel physiological functions of pro-apoptotic cysteine proteases in endochondral ossification</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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