Wnt Signalling in Ameloblastoma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F21%3A43879438" target="_blank" >RIV/62157124:16170/21:43879438 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Wnt Signalling in Ameloblastoma
Original language description
The wingless-related integration site pathway (WNT) is crucial for tissue homeostasis and its misregulation was described in numerous diseases including cancer. We focused on the expression and possible alteration of WNT signalling pathway in human ameloblastoma - the most common oral tumor with an odontogenic origin. Ameloblastoma is a benign tumor, but its invasive behaviour often makes the treatment complicated. We used immunohistochemistry and immunofluorescence to map the expression of key WNT members and PCR array to analyze gene expression of 84 WNT members in ameloblastoma samples. Our analysis revealed that members of canonical WNT signaling pathway such as EP300 and TCF7 were significantly upregulated. The most up regulated receptors in ameloblastoma were FZD1, FZD7 and co-receptor LRP5. On the other hand, our analysis also revealed significant upregulation of several genes associated with inhibition of WNT signaling such as SFRP1, or NLK in ameloblastoma. A number of WNT signaling target genes were also affected, with PITX2 and AXIN2 significantly upregulated and FOSL1 downregulated. Two planar cell polarity genes, VANGL2 and MAPK8 were upregulated, while DAAM1 was downregulated in ameloblastoma when compared to gingival tissues. In conclusion, our study provided deeper insight into gene expression profile of WNT pathway in ameloblastoma, opening up new avenues which should be tested for potential prediction of future cell behaviour in this neoplasm.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů