Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880154" target="_blank" >RIV/62157124:16170/22:43880154 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/22:43880154

Result on the web

<a href="https://www.mdpi.com/2306-7381/9/2/35" target="_blank" >https://www.mdpi.com/2306-7381/9/2/35</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/vetsci9020035" target="_blank" >10.3390/vetsci9020035</a>

Result language

angličtina

Original language name

Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters

Original language description

The aim of this study was the direct detection of feline coronavirus by real-time PCR and by three different rapid immunochromatographic (RIM) tests detecting antigens in faecal samples of shelter cats. Based on sensitivity and specificity calculated for each of the RIM tests, the utility of RIM tests was compared. Seventy faecal samples originating from shelter cats housed in quarantine were examined. Out of 70 samples analyzed by real-time PCR, 44 (62.9%) were positive. Significantly more cats (p &lt; 0.05) tested positive than negative. Neither age nor sex of the cats played a significant role (p &gt; 0.05) in the shedding status of the virus. The sensitivity of the RIM tests was found to be at low (&lt;35%; RIM tests A and C) to satisfactory level (&gt;50%, RIM test B). The number of virus particles determined by real-time RT-PCR analysis did not significantly correlate with the results detected by any of the RIM tests (p &gt; 0.05). The results of this study indicate that the use of rapid antigen RIM tests in routine screening of FCoV shedding status in shelter cats is limited due to the occurrence of a high number of false negative results.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Sciences

ISSN

2306-7381

e-ISSN

2306-7381

Volume of the periodical

9

Issue of the periodical within the volume

2

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

nestrankovano

UT code for WoS article

000762017700001

EID of the result in the Scopus database

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