Characterization of flavobacterium psychrophilum isolates form Czech aquaculture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880296" target="_blank" >RIV/62157124:16170/22:43880296 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Characterization of flavobacterium psychrophilum isolates form Czech aquaculture

Original language description

Flavobacterium psychrophilum is an important fish pathogen causing economical losses mainly in salmonids all over the world, including the Czech Republic. Several studies have characterized F. psychrophilum isolates from various countries, but none from the Czech Republic. Aim of this studywas to characterize isolates gained in 2012-2019 from aquaculture in the Czech Republic. Seventy isolates from 11 farms were included in this study. Analysis of antimicrobial susceptibility to most frequently used antibiotics in aquaculture using broth microdilution method, presence of selected virulence factors using culture methods and serotyping using mPCR protocol was performed. Antimicrobial susceptibility testing showed high MIC for oxolinic acid, oxytetracycline, flumequine, enrofloxacin and increasing resistance to florfenicol, which is currently drug of choice for flavobacteriosis treatment in many countries. By mPCR-based serotyping type 1, corresponding to serotype Fd, was most frequently identified, less often type 2, corresponding to serotype Th, and only sporadically types 3 and 4, corresponding to serotype FpT. No type 0 was detected. All isolates were able to create visible biofilm, high proportion created sediment and hydrolyzed gelatin, but only one third of isolates was positive for gliding motility. Characterization of isolates is essential for revealing ways of introduction of the pathogen in the farms and enables to create effective protective measures in the aquaculture. This study showed increasing resistance to antibiotics similarly as in ther countries, meaning that alternative therapy methods are necessary to be studied.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů