Comparison of two methods of measuring the urinary protein concentration for the determination of the urinary protein to creatinine ratio in various animal species

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F24%3A43881722" target="_blank" >RIV/62157124:16170/24:43881722 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/24:43881722

Result on the web

<a href="https://vetmed.agriculturejournals.cz/artkey/vet-202408-0003_comparison-of-two-methods-of-measuring-the-urinary-protein-concentration-for-the-determination-of-the-urinary-p.php" target="_blank" >https://vetmed.agriculturejournals.cz/artkey/vet-202408-0003_comparison-of-two-methods-of-measuring-the-urinary-protein-concentration-for-the-determination-of-the-urinary-p.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/30/2024-VETMED" target="_blank" >10.17221/30/2024-VETMED</a>

Result language

angličtina

Original language name

Comparison of two methods of measuring the urinary protein concentration for the determination of the urinary protein to creatinine ratio in various animal species

Original language description

Determination of the urinary protein-to-creatinine ratio (UPC) is an important tool in the quantification of proteinuria in animals. However, the result may be affected by the different methods of determining the urinary protein concentration. The aim of this study was to compare the turbidimetric method using benzethonium chloride and the colorimetric method using pyrogallol red in the measurement of the urinary protein concentration in dogs, cats, guinea pigs and horses. A total of 464, 192, 216 and 119 urine samples from dogs, cats, guinea pigs and horses were examined in the study, respectively. The group consisted of animals of both sexes and different ages, and, in the dogs and cats, it included both healthy animals and those with various health problems. In the group of horses and guinea pigs, only clinically healthy animals were included. A total of 347, 185, 103 and 100 samples from the dogs, cats, guinea pigs and horses were used in the statistical analysis; the other values were excluded as they were below the detection limit. According to the Passing-Bablok analysis, there was a significant constant and proportional difference in the horses. In the dogs, cats and guinea pigs, there was a significant constant difference, but no proportional difference. The Bland-Altman method showed significant bias between the two methods in the horses and cats, but not in the dogs and guinea pigs. In the dogs and cats, the agreement between the two methods was tested and expressed as Cohen&apos;s kappa (x). In the cats, it was almost perfect for the proteinuric samples (x = 0.823 3) and significant for the non-proteinuric samples (x = 0.804 9). In the dogs, the agreement was significant for the non-proteinuric samples (x = 0.621 5) and only moderate for the proteinuric samples (x = 0.527 5). The influence of the method used to determine the urinary protein concentration should be taken into account when evaluating the UPC. Repeated examinations in one patient should be performed with the same method.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

1805-9392

Volume of the periodical

69

Issue of the periodical within the volume

8

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

273-280

UT code for WoS article

001306017500002

EID of the result in the Scopus database

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