Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F08%3A00001706" target="_blank" >RIV/62157124:16270/08:00001706 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12610/08:00008882
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca
Original language description
Glutathione belongs to a vital intra- and extra-cellular protective antioxidant and is found almost exclusively in its reduced form. The ratio between its reduced and oxidized within cells is often used as a marker of cellular toxicity. The objectives ofthe study were to (i) determine both the reduced (GSH) and oxidized glutathione (GSSG) and cysteine (Cys) in the sperm of the Acipenser baerii and Acipenser ruthenus, as well as in Perca fluviatilis and Sander lucioperca, and (ii) to demonstrate the differences in concentration levels between representatives of acipenseriform and teleost species. The average content of the thiols determined in the sperm samples were as follows: Acipenser baerii (cysteine 55 +-; 8 lg ml)1; GSH 126 +-; 19 lg ml)1; GSSG 49 +-; 7 lg ml)1), Acipenser ruthenus (cysteine 62 +-; 9 lg ml)1; GSH 768 +- 115 lg ml)1; GSSG 180 +-; 16 lg ml)1), Sander lucioperca (cysteine 251 +- 38 lg ml)1; GSH 185 +-; 28 lg ml)1; GSSG 93 +-; 14 lg ml)1), Perca fluviatilis (cysteine
Czech name
Obsah redukovaného a oxidovaného glutathionu ve spermatu Acipenser baerii and A. ruthenus a Perca fluviatilis and Sander lucioperca
Czech description
Zájmem této práce bylo určit redukovaný a oxidovaný glutathion a cystein ve spermatu Jesetera sibiřského, Jesetera malého, Okouna říčního a Candáta obecného. Zaznamenali jsme rozdíli mezi těmi to druhy ryb. Průměrný obsah thiolů stanovena ve vzorku byl:Acipenser baerii (cystein 55 + -; 8 lg ml) 1; GSH 126 + -; LG 19 ml) 1; GSSG 49 + -, 7 ml lg) 1 ), Acipenser ruthenus (cystein 62 + -; 9 lg ml) 1; GSH 768 + - 115 ml lg) 1; GSSG 180 + -; LG 16 ml) 1), Sander lucioperca (cystein 251 + - 38 lg ml) 1 ; GSH185 + -; LG 28 ml) 1; GSSG 93 + -; LG 14 ml) 1), Perca fluviatilis (cystein 281 + -; LG 42 ml) 1; GSH 496 + -; LG 74 ml) 1; GSSG 138 + -; LG 21 ml) 1). Na základě výsledků získaných lze dospět k závěru, že tato metoda je citlivý a selektivní pro stanovení těchto látek v reálných vzorcích. Výsledky odhalily rozdíly v obsahu cystein mezi druhy ze dvou systematických kategorií, ale také ukázala, že poměr mezi GSH a GSSG se může lišit mezi druhy.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Ichthyology
ISSN
0175-8659
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
3
Pages from-to
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UT code for WoS article
000258458400026
EID of the result in the Scopus database
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