Complete sequencing of an IncHI1 plasmid encoding the carbapenemase NDM-1, the ArmA 16S RNA methylase and a resistance-nodulation-cell division/multidrug efflux pump
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F13%3A43872420" target="_blank" >RIV/62157124:16270/13:43872420 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/jac/dks357" target="_blank" >http://dx.doi.org/10.1093/jac/dks357</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jac/dks357" target="_blank" >10.1093/jac/dks357</a>
Alternative languages
Result language
angličtina
Original language name
Complete sequencing of an IncHI1 plasmid encoding the carbapenemase NDM-1, the ArmA 16S RNA methylase and a resistance-nodulation-cell division/multidrug efflux pump
Original language description
To characterize the pNDM-CIT plasmid identified in Citrobacter freundii carrying genes encoding the metallo--lactamase NDM-1 and the 16S RNA methylase ArmA. The complete DNA sequence of pNDM-CIT was obtained by using the 454-Genome Sequencer FLX procedure on a library obtained using plasmid DNA purified from the pNDM-CIT Escherichia coli J53 transconjugant. Contig assembly and predicted gaps were confirmed and filled by PCR-based gap closure. Comparative analysis with IncHI1 incompatibility group plasmids was performed using BLASTN and BLASTP algorithms. Plasmid pNDM-CIT was 288920 bp and revealed an IncHI1 plasmid scaffold, showing novel resistance and potential virulence determinants. The bla(NDM-1) gene was identified within a novel genetic context,flanked by a duplication of the class 1 integron on both sides. The replicase gene repAciN, originating from Acinetobacter spp. plasmids, was identified in a close association with the Tn1548::armA transposon and the macrolide resistance
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Antimicrobial Chemotherapy
ISSN
0305-7453
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
34-39
UT code for WoS article
000312646300007
EID of the result in the Scopus database
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