Brain is the predilection site of Toxoplasma gondii in experimentally inoculated pigs as revealed by magnetic capture and real-time PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F14%3A43871838" target="_blank" >RIV/62157124:16270/14:43871838 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/14:43871838 RIV/62157124:16810/14:43871838

Result on the web

<a href="http://dx.doi.org/10.1016/j.fm.2013.08.011" target="_blank" >http://dx.doi.org/10.1016/j.fm.2013.08.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fm.2013.08.011" target="_blank" >10.1016/j.fm.2013.08.011</a>

Result language

angličtina

Original language name

Brain is the predilection site of Toxoplasma gondii in experimentally inoculated pigs as revealed by magnetic capture and real-time PCR

Original language description

Pigs represent an important source of food in many countries, and undercooked pork containing tissue cysts is one of the most common sources of Toxoplasma gondii infection for humans. A magnetic capture method for the isolation of T. gondii DNA and quantitative real-time PCR targeting the 529 bp TOXO repeat element were used to estimate the parasite burden in different tissues of pigs experimentally infected with T. gondii oocysts, and to determine the predilection sites of T. gondii in this host species. The highest concentration of T. gondii DNA was found in brain tissues, equivalent to [median] 553.7 (range 3857.7e121.9) parasites per gram, followed by lungs, heart and dorsal muscles with median values corresponding to 0.3 (range 61.3-0.02); 2.6 (range 7.34-0.37) and 0.6 (range 2.81-0.31) parasites per gram of tissue, respectively. Skeletal muscles from fore and hindlimb, liver and kidney presented very low infection burdens equivalent to [median] _0.2 parasites per gram of tissues,

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food Microbiology

ISSN

0740-0020

e-ISSN

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Volume of the periodical

38

Issue of the periodical within the volume

APR 2014

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

167-170

UT code for WoS article

000328660400022

EID of the result in the Scopus database

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