Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873359" target="_blank" >RIV/62157124:16270/15:43873359 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16170/15:43873359 RIV/62157124:16810/15:43873359
Result on the web
<a href="http://dx.doi.org/10.1016/j.fm.2015.07.005" target="_blank" >http://dx.doi.org/10.1016/j.fm.2015.07.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fm.2015.07.005" target="_blank" >10.1016/j.fm.2015.07.005</a>

Result language
angličtina
Original language name
Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection
Original language description
Undercooked lamb and mutton are common sources of Toxoplasma gondii infection for humans. A sequence specific magnetic capture technique in combination with quantitative real-time PCR targeting the 529 bp repeat element of T. gondii was used for estimation of the parasite burdens in various sheep tissues (n = 6) three months after peroral experimental inoculation with 10,000 T. gondii oocysts. Brain was the most frequently affected organ (positive in all 6 sheep) and showed the highest estimated parasite loads (0.5-30,913 parasites/g tissue). Lung samples were positive in three sheep, with load estimates of 36.3 to < 1 parasite/g tissue. Heart tissue was positive in three sheep and kidney only in one animal with low parasite loads (< 1 parasite/g tissue). Only few skeletal muscle samples in 2 animals showed positive results, with very low parasite burdens, while samples from further internal organs (i.e. liver and spleen) were negative in all animals. This study identified the brain as
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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