The impact of processing technologies and isolation procedures on the quality of animal DNA in food and feed products
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F17%3A43875937" target="_blank" >RIV/62157124:16270/17:43875937 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The impact of processing technologies and isolation procedures on the quality of animal DNA in food and feed products
Original language description
The aim of this study was to find suitable methods for the homogenization and isolation of nucleic acids from samples of both raw and thermally or mechanically treated muscle tissue and different types of meat products and feeds. The effect of different processing methods (high temperature, pressure, mechanical processing, addition of other raw materials) on DNA from animal tissues (chicken, pork, beef) in products manufactured in technology workshops was investigated. Subsequently, several extraction techniques for DNA isolation from model meat products were tested. DNA quality and quantity were assessed by absorbance measurements and determination of the ratio of absorbance using a spectrophotometer. Gel electrophoresis was performed in order to determine the degree of DNA fragmentation induced by technological processing. The amplification ability was tested by PCR analysis.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
21101 - Food and beverages
Result continuities
Project
<a href="/en/project/QJ1530107" target="_blank" >QJ1530107: Methods for identification, traceability and authenticity verification of feed mixtures and food containing components of animal origin</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XIX. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ Sborník příspěvků
ISBN
978-80-7305-788-6
ISSN
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e-ISSN
neuvedeno
Number of pages
3
Pages from-to
21-23
Publisher name
Veterinární a farmaceutická univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
May 24, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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