Detection of fluoroquinolone residues in milk using Yersinia spp. strains: towards better sensitivity for flumequine determination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F17%3A43876204" target="_blank" >RIV/62157124:16270/17:43876204 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1515/jvetres-2017-0061" target="_blank" >http://dx.doi.org/10.1515/jvetres-2017-0061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/jvetres-2017-0061" target="_blank" >10.1515/jvetres-2017-0061</a>

Result language

angličtina

Original language name

Detection of fluoroquinolone residues in milk using Yersinia spp. strains: towards better sensitivity for flumequine determination

Original language description

Plate diffusion methods play an important role in the monitoring system for antimicrobial agents in raw materials and foodstuffs of animal origin. The aim of this work was to select a Yersinia spp. strain for the plate diffusion method based on sensitivity to a fluoroquinolone, namely flumequine. Another aim was to determine the optimal conditions of the method with the selected strain of Yersinia ruckeri CCM 8467 and to determine the detection capability (CC?) of this method for residues of selected fluoroquinolones in milk. Material and Methods: Optimum method conditions were set: cell concentration in the test agar at the level of 9.105?106 CFU/mL, discs with a diameter of 12.7 mm, Antimicrobial Inhibitor Test Agar with a pH of 6.0, and incubation at 30°C for at least 18 h and up to 24 h. Results: With respect to the maximum residue limit (MRL), the Y. ruckeri plate method demonstrated the lowest sensitivity to flumequine. The CC? of the method for flumequine was in the concentration of 100 ?g/L (twice the MRL). The study also confirmed that the method exhibits very good sensitivity to the other tested fluoroquinolones, which were marbofloxacin (30 ?g/L, 0.4 MRL), ciprofloxacin (10 ?g/L, 0.1 MRL), and enrofloxacin (20 ?g/L, 0.2 MRL), but lower sensitivity to danofloxacin (42 ?g/L, 1.4 MRL). Conclusion: The method with the CCM 8467 strain of Y. ruckeri showed a higher sensitivity to flumequine than the method with the ATCC 11303 strain of E. coli.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/QJ1230044" target="_blank" >QJ1230044: Determination of parameters for legislative assessing of the quality and safety of raw milk from cows, sheep and goats</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Veterinary Research

ISSN

2450-7393

e-ISSN

—

Volume of the periodical

61

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

467-472

UT code for WoS article

000418995600014

EID of the result in the Scopus database

2-s2.0-85042924965