Development of methods for Dermacentor reticulatus and D. marginatus cross-amplification study

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877964" target="_blank" >RIV/62157124:16270/19:43877964 - isvavai.cz</a>
Result on the web
<a href="https://docplayer.cz/147890435-Xxi-konference-mladych-vedeckych-pracovniku-s-mezinarodni-ucasti-sbornik-prispevku.html" target="_blank" >https://docplayer.cz/147890435-Xxi-konference-mladych-vedeckych-pracovniku-s-mezinarodni-ucasti-sbornik-prispevku.html</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Development of methods for Dermacentor reticulatus and D. marginatus cross-amplification study
Original language description
There are two species of Dermacentor genera that are endemic to Europe, Dermacentor reticulatus (Fabricius, 1974) and Dermacentor marginatus (Sulzer, 1776). D. reticulatus is the second most abundant species in central Europe, after Ixodes ricinus, with Southern Europe as the southern limit of the distribution. D. marginatus is a frequent tick in the Mediterranean region. There is the number of pathogens that these species can transmit or at least carry. To better understand gene flow and transmission of pathogens we designed 17 polymorphic microsatellites loci for D. reticulatus species. All loci were analyzed using DNA isolated from ticks collected by the standard flagging method. In order to determinate the success rate of cross-species microsatellite amplification, we tested all microsatellite loci on D. marginatus collected from 7 localities in 4 countries. We managed to amplify five of them in specimens of D. marginatus, 3 dinucleotide and 2 trinucleotide.
Czech name
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Czech description
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Project
<a href="/en/project/NV16-33934A" target="_blank" >NV16-33934A: Hidden threat of natural foci of understudied tick-borne infections. Case of the genera Rickettsia, Anaplasma, Babesia</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XXI. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ, Sborník příspěvků
ISBN
978-80-7305-820-3
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
74-76
Publisher name
Veterinární a farmaceutická univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
May 22, 2019
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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