Antimicrobial activity of natural soaps tested by Bioscreen methodology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F20%3A43878634" target="_blank" >RIV/62157124:16270/20:43878634 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/20:00115610

Result on the web

<a href="http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1119" target="_blank" >http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1119</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.30970/sbi.1401.608" target="_blank" >10.30970/sbi.1401.608</a>

Result language

angličtina

Original language name

Antimicrobial activity of natural soaps tested by Bioscreen methodology

Original language description

The aim of this study was to combine the utilization of waste frying oils within soap making process in order to make useful and environmentally friendly solutions and development of methods for determination of the antimicrobial effect of those created products. Soaps were made from edible oils which are fried in laboratory conditions. The antimicrobial activity of soaps was done against Staphylococcus aureus species as one of the representatives of the human skin microbiome. Two methods were applied: agar dilution method and the method including kinetics following on Bioscreen microbiology reader. In the first method, the number of CFU was followed on agar medium with and without different soap solutions after incubation for 24 hours at 30 °C. The result for IC50 (inhibition concentration for 50% of population) was 0.08 mg/mL. Minimal inhibition concentration was detected at 0.41 mg/mL and minimal bactericidal concentration was observed at &gt; 0.75 mg/mL for selected soap solution. Soap concentrations of 0.3 mg/mL of soaps (made from fresh and fried oil) were used for Bioscreen assessment with measurement on every hour during the 7 hours of incubation at 30 °C. 5-second sequence of shaking of the microplate was applied before each measurement which was done at the wavelength of 610 nm. The growth coefficients of the culture with soap solutions added and from the growth of culture only were compared. The growth of S. aureus subjected to soaps made from fresh and fried oils was inhibited 55.3% and 69.7% respectively against the control during the first seven hours of incubation. From results obtained, it was concluded that there is a great potential of the Bioscreen as a method for further studies on antibacterial activity of soaps made from waste frying oils.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

21101 - Food and beverages

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Studia Biologica

ISSN

1996-4536

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

1

Country of publishing house

UA - UKRAINE

Number of pages

10

Pages from-to

23-32

UT code for WoS article

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EID of the result in the Scopus database

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