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Non-invasive assessment of adrenocortical activity as a measure of stress in ring-tailed lemurs (Lemur catta)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F20%3A43878787" target="_blank" >RIV/62157124:16270/20:43878787 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.vetline.de/non-invasive-assessment-of-adrenocortical-activity-as-a-measure-of-stress-in-ring-tailed-lemurs" target="_blank" >https://www.vetline.de/non-invasive-assessment-of-adrenocortical-activity-as-a-measure-of-stress-in-ring-tailed-lemurs</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.2376/0005-9366-18078" target="_blank" >10.2376/0005-9366-18078</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Non-invasive assessment of adrenocortical activity as a measure of stress in ring-tailed lemurs (Lemur catta)

  • Original language description

    The non-invasive assessment of adrenocortical activity by measuring faecal glucocorticoid metabolites (FGMs) in faeces is currently a widely used method, especially in exotic animals. The aim of our study was to biologically validate the suitability of six different enzyme immunoassays (EIAs) to quantify changes in FGM concentrations after a known strong stress stimulus (capture, sedation, blood collection for serological examination and tuberculination) in ring-tailed lemurs (Lemur cotta). Faecal samples were collected from six animals (four males and two females) twice daily spanning from two days before until three days after the exposure to the stress stimulus. The faecal samples obtained were extracted and the supernatants were measured for immunoreactive FGM concentrations using six different EIAs, namely cortisol, corticosterone, 11-oxoaetiocholanolone 1, 11-oxoaetiocholanolone II, 11 beta-hydroxyaetiocholanolone and 5a-pregnane-3 beta,11 beta,21-triol-20-one EIA. The baseline levels, peak levels (absolute concentration and percentage increase), the duration of the peak and lag time of the peak were calculated and statistically evaluated. The FGM concentrations started to increase on average 23 hours after the exposure to the stress stimulus and returned to pre-stress levels on average within 36 hours. Our results suggest that the two group-specific 11-oxoaetiocholanolone EIAs and the 11 beta-hydroxyaetiocholanolone assay are generally suitable for the monitoring of stress response in ring-tailed lemurs.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40301 - Veterinary science

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Berliner und Münchener Tierärztliche Wochenschrift

  • ISSN

    0005-9366

  • e-ISSN

  • Volume of the periodical

    133

  • Issue of the periodical within the volume

    1-2

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    8

  • Pages from-to

    28-35

  • UT code for WoS article

    000516881300004

  • EID of the result in the Scopus database