IncX plasmids and their transferability under induced stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879712" target="_blank" >RIV/62157124:16270/21:43879712 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16810/21:43879712

Result on the web

<a href="https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf" target="_blank" >https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

IncX plasmids and their transferability under induced stress

Original language description

Dissemination of genes encoding for antibiotic resistance is mediated predominantly by the conjugation of plasmids. Here we focus on the IncX1 and IncX2 group of plasmids carrying genes conferring plasmid-mediated quinolone resistance (PMQR), presenting trends of their transfer under induced stress of temperature change and antibiotic supplementation. In two representatives of IncX1 (pHP2) and IncX2 (p194) plasmids and their deletion mutants in qnrS gene (pHP2 ?qnrS, p194 ?qnrS), the influence of induced stress on conjugative transfer was investigated. A deletion of the qnr gene was observed to modify the frequency of plasmid transfer. In order to affect the transfer rate of wildtype plasmids, the influence of two stresses simultaneously was needed, whereas the single stress was sufficient to affect the IncX ?qnrS plasmid transfer rate. This study showed the significant properties and behaviours of IncX plasmids carrying antibiotic resistance genes that are likely to play a role in their dissemination in bacterial populations.Dissemination of genes encoding for antibiotic resistance is mediated predominantly by the conjugation of plasmids. Here we focus on the IncX1 and IncX2 group of plasmids carrying genes conferring plasmid-mediated quinolone resistance (PMQR), presenting trends of their transfer under induced stress of temperature change and antibiotic supplementation. In two representatives of IncX1 (pHP2) and IncX2 (p194) plasmids and their deletion mutants in qnrS gene (pHP2 ?qnrS, p194 ?qnrS), the influence of induced stress on conjugative transfer was investigated. A deletion of the qnr gene was observed to modify the frequency of plasmid transfer. In order to affect the transfer rate of wildtype plasmids, the influence of two stresses simultaneously was needed, whereas the single stress was sufficient to affect the IncX ?qnrS plasmid transfer rate. This study showed the significant properties and behaviours of IncX plasmids carrying antibiotic resistance genes that are likely to play a role in their dissemination in bacterial populations.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA18-23532S" target="_blank" >GA18-23532S: Multiresistant high-risk Escherichia coli lineages: a success story of the chromosome and plasmid interplays</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník příspěvků XXIII. konference mladých vědeckých pracovníků s mezinárodní účastí

ISBN

978-80-7305-851-7

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

96-98

Publisher name

Veterinární univerzita Brno

Place of publication

Brno

Event location

Brno

Event date

Jun 14, 2021

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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