Use of the fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA) for the detection of oxidative stress in dried plant matrices

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881464" target="_blank" >RIV/62157124:16270/24:43881464 - isvavai.cz</a>
Result on the web
<a href="https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf" target="_blank" >https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Use of the fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA) for the detection of oxidative stress in dried plant matrices
Original language description
The aim of this work was to detect the level of total oxidative stress in dried plant matrices. Dried leaves of green barley and matcha green tea in powder form were selected for analysis. The samples, fixed on a slide using glycerol gelatin, were incubated with 2',7'-dichlorofluorescin diacetate (DCFH-DA) fluorescent probe for 30 min without light access and then analyzed using Leica DM5500 microscope with Leica K5 fluorescence camera and λex 504 nm; λem 524 nm fluorescence cube. The analysis showed significant differences (p<0.05) between the control group of dried green barley sample and the group of samples treated with the fluorescent probe, a significant difference (p<0.05) was also observed in the case of barley and matcha tea samples with probe application. However, in the case of matcha green tea, no significant differences were observed between the samples. The 2',7'-dichlorofluorescin diacetate (DCFH-DA) fluorescent probe appears to be a sensitive method for the determination of total ROS levels in plant samples, also suitable for dried green barley matrix.
Czech name
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Czech description
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Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XXVI. konference mladých vědeckých pracovníků s mezinárodní účastí: Sborník příspěvků
ISBN
978-80-7305-957-6
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
5-7
Publisher name
Veterinární univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
Jun 5, 2024
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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