Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F15%3A43873465" target="_blank" >RIV/62157124:16370/15:43873465 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26220/14:PU109573 RIV/00159816:_____/15:00061108 RIV/62156489:43210/15:43906311

Result on the web

<a href="http://dx.doi.org/10.1016/j.chemosphere.2014.05.074" target="_blank" >http://dx.doi.org/10.1016/j.chemosphere.2014.05.074</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2014.05.074" target="_blank" >10.1016/j.chemosphere.2014.05.074</a>

Result language

angličtina

Original language name

Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Original language description

Physiological responses of Scenedesmus quadricauda to Cr(VI) and Cr(III) excess were studied in buffer with circumneutral pH (6.5). Total Cr content was similar in low (1 mu M of both oxidation states) but higher in 10 WO Cr(VI) treatment and high accumulation potential was detected (80-82% and 41-65% in 1 and 10 mu M treatments, respectively). Specific fluorescence indicator (6-((anthracen-9-yl)methyleneamino)-2H-chromen-2-one) confirmed partial reduction of Cr(VI) to Cr(III) under exposure conditions. Viability and chlorophyll autofluorescence were more depleted by Cr(VI) while Cr(III) stimulated increase in ROS and lipid peroxidation. Antioxidative enzyme activities showed significantly higher values in 10 mu M treatments of both Cr oxidation states. Depletion of mitochondrial proteins was not reflected in alteration of total soluble proteins indicating sensitivity of this organelle to Cr and TTC test showed no clear oxidation state-related effect. In this view, "Cr(VI) is not more toxic than Cr(III)" at least for some parameters. Subsequent study with the application of 10 mu M Cr(VI) confirmed that HEPES buffer is more suitable exposure solution for toxicological studied than water or inorganic salts (higher chlorophyll autofluorescence was observed) and pH 6.5 is more suitable than low or high pH (4.5 or 8.5) in terms of Cr uptake. Another known Cr(III) fluorescence indicator (naphthalimide rhodamine) also confirmed partial reduction of Cr(VI) to Cr(III) at acidic pH but only traces were seen at alkaline pH.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemosphere

ISSN

0045-6535

e-ISSN

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Volume of the periodical

120

Issue of the periodical within the volume

feb

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

23-30

UT code for WoS article

000348003200004

EID of the result in the Scopus database

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