Endophytic Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F16%3A43874158" target="_blank" >RIV/62157124:16370/16:43874158 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10295-016-1800-4" target="_blank" >http://dx.doi.org/10.1007/s10295-016-1800-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10295-016-1800-4" target="_blank" >10.1007/s10295-016-1800-4</a>

Result language
angličtina
Original language name
Endophytic Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins
Original language description
In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magneticresonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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