Proteomic analysis of the vitamin C effect on the doxorubicin cytotoxicity in the MCF-7 breast cancer cell line

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F17%3A43876021" target="_blank" >RIV/62157124:16370/17:43876021 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00432-016-2259-4" target="_blank" >http://dx.doi.org/10.1007/s00432-016-2259-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00432-016-2259-4" target="_blank" >10.1007/s00432-016-2259-4</a>

Result language

angličtina

Original language name

Proteomic analysis of the vitamin C effect on the doxorubicin cytotoxicity in the MCF-7 breast cancer cell line

Original language description

Purpose Doxorubicin is an anthracycline drug which inhibits the growth of breast cancer cell lines. However, a major factor limiting its use is a cumulative, dose-dependent cardiotoxicity, resulting in a permanent loss of cardiomyocytes. Vitamin C was found to potentiate the cytotoxic effects of a variety of chemotherapeutic drugs including doxorubicin. The aim of the study was to describe the changes in protein expression and proliferation of the MCF-7 cells induced by the vitamin C applied with doxorubicin. Methods Label-free quantitative proteomics and real-time cell analysis methods were used to search for proteome and cell proliferation changes. These changes were induced by the pure DOX and by DOX combined with vitamin C applied on the MCF-7 cell line. Results From the real-time cell analysis experiments, it is clear that the highest anti-proliferative effect occurs with the addition of 200 mu M of vitamin C to 1 mu M of doxorubicin. By applying both the label-free protein quantification method and total ion current assay, we found statistically significant changes (p &lt;= 0.05) of 26 proteins induced by the addition of vitamin C to doxorubicin on the MCF-7 cell line. These differentially expressed proteins are involved in processes such as structural molecule activity, transcription and translation, immune system process and antioxidant, cellular signalling and transport. Conclusion The detected proteins may be capable of predicting response to DOX therapy. This is a key tool in the treatment of breast cancer, and the combination with vit C seems to be of particular interest due to the fact that it can potentiate anti-proliferative effect of DOX.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cancer Research and Clinical Oncology

ISSN

0171-5216

e-ISSN

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Volume of the periodical

143

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

35-42

UT code for WoS article

000392629700004

EID of the result in the Scopus database

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