Cognate PXR ligands tributyltin and triphenyltin isothiocyanates induce DNA crosslinks ? evoked apoptosis in human breast carcinoma MCF 7 and MDA-MB-231 cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F18%3A43877159" target="_blank" >RIV/62157124:16370/18:43877159 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cognate PXR ligands tributyltin and triphenyltin isothiocyanates induce DNA crosslinks ? evoked apoptosis in human breast carcinoma MCF 7 and MDA-MB-231 cell lines
Original language description
Two freshly synthesized triorganotin isothiocyanate compounds (commercially inavailable), cognate nuclear retinoid X receptor ligands, were tested in estrogen receptor positive MCF 7 and negative MDA-MB-231 human breast carcinoma cell lines. Cytotoxicity measured by MTT assay has shown tributyltin isothiocy to be more cytotoxic than triphenyltin isothiocyanate in MDA-MB-231 cell ine. Comet assay revealed the presence of crosslinks after the treatment wit derivatives. Differences in cytotoxicity of both compounds detected by FDA staining corresponded to the MTT data, indicating more pronounced effects in MCF 7 than in MDA-MB-231 cell line. Both derivatives caused apoptosis by intrinsic pathway, as shown by the mitochondrial membrane potential (MMP) depolarization an caspase-3/7 activation. The onset of caspase activation correlated with MMP dissipation and the tota cytotoxicity more than the amount of active caspases. In conclusion, our data suggest that the induced by tributyltin isothiocyanate and triphenyltin isothiocyanate treatment is the main cause of their cytotoxicity in cell lines studied. 13 1742/5000
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30107 - Medicinal chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů