Beta-lactamase producing Escherichia coli and antibiotic resistance genes in municipal and hospital wastewaters

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F19%3A43878234" target="_blank" >RIV/62157124:16810/19:43878234 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Beta-lactamase producing Escherichia coli and antibiotic resistance genes in municipal and hospital wastewaters

Original language description

Wastewaters represent environment where sewage from different sources is interacting and are considered the hot spots of antimicrobial resistance (AMR). In this study, level of AMR in municipal (outflow/inflow to/from wastewater treatment plant (WWTP)), hospital (inflow/outflow to/from hospital WWTP and raw sewage from hospital) and river waters in the city of Brno (Czech Republic) was examined. Total vs cefotaxime (CTX)-resistant E. coli were enumerated in all samples. Based on phenotypic and genotypic results, representative CTX-resistant isolates and whole-community DNA were subjected to sequencing (Illumina) and bioinformatics analysis. In total 95.6% (n=158) of CTX-resistant E. coli were extended-spectrum beta-lactamase (ESBL) producers and were detected in all samples except the outflow from hospital WWTP. Most isolates were positive for gene blaCTX-M (98.7%; n=151) encoding ESBL. The metagenomic analysis showed high prevalence of pathogenic bacteria in hospital WWs and majority of non-pathogenic bacteria in municipal WWs, treated and river waters. Genes encoding resistance to aminoglycosides, beta-lactams and macrolides were frequently detected, however blaCTX-M was not found in this metagenomic dataset. The study confirmed municipal treated water as source of multi-resistant E. coli and AMR genes for the environment. Moreover, the combination of two different approaches brought complex view on AMR in water environment.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů