Deproteinization is necessary for the accurate determination of ammonia levels by glutamate dehydrogenase assay in blood plasma from subjects with liver injury

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18450%2F17%3A50013791" target="_blank" >RIV/62690094:18450/17:50013791 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/17:10365014
Result on the web
<a href="http://dx.doi.org/10.1093/labmed/lmx053" target="_blank" >http://dx.doi.org/10.1093/labmed/lmx053</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/labmed/lmx053" target="_blank" >10.1093/labmed/lmx053</a>

Result language
angličtina
Original language name
Deproteinization is necessary for the accurate determination of ammonia levels by glutamate dehydrogenase assay in blood plasma from subjects with liver injury
Original language description
Objective: To determine the effect of presence of high concentrations of nicotinamide adenine dinucleotide (NADH)– and nicotinamide adenine dinucleotide phosphate (NADPH)–consuming enzymes on the accuracy of glutamate dehydrogenase (GLDH) assay for ammonia. Methods: We measured ammonia concentrations using GLDH and NADH or NADPH in blood-plasma specimens and specimens deproteinized by sulfosalicylic acid from CCl4-treated or control rats. The nonspecific oxidation of NADH and NADPH was measured in mixtures without GLDH. Results: We observed a gradual decrease (~0.5%) in absorbance in the plasma of controls after the addition of NADH but not after adding NADPH. The decrease in absorbance in plasma of CCl4-treated animals was 13.2% and 5.2% after the addition of NADH and NADPH, respectively. The decrease in absorbance was not detected in deproteinized specimens. The values of ammonia concentration were higher in the plasma specimens compared with the deproteinized ones. Conclusion: Deproteinization is necessary for accurate measurement of ammonia using GLDH assay in the blood plasma of subjects with liver injury.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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