A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F13%3A50001708" target="_blank" >RIV/62690094:18470/13:50001708 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0278691513006480" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0278691513006480</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fct.2013.09.010" target="_blank" >10.1016/j.fct.2013.09.010</a>
Alternative languages
Result language
angličtina
Original language name
A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic
Original language description
Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0,037 to 1,130 ug/L. The outcome of ELISA and HPLC measurements were well correlated (r = 0.907). However, it was observed that ELISA, tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050ug/L under standard operation conditions. W
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food and chemical toxicology
ISSN
0278-6915
e-ISSN
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Volume of the periodical
62
Issue of the periodical within the volume
12.09.2013
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
427-431
UT code for WoS article
000329960400056
EID of the result in the Scopus database
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