Simvastatin Inhibits Endotox-inInduced Apoptosis in Liver and Spleen Through Up-Regulation of Survivin/NF-kappa B/p65 Expression

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F19%3A50015493" target="_blank" >RIV/62690094:18470/19:50015493 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/19:10395568 RIV/00179906:_____/19:10395568

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fphar.2019.00054/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphar.2019.00054/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphar.2019.00054" target="_blank" >10.3389/fphar.2019.00054</a>

Result language

angličtina

Original language name

Simvastatin Inhibits Endotox-inInduced Apoptosis in Liver and Spleen Through Up-Regulation of Survivin/NF-kappa B/p65 Expression

Original language description

Endotoxemia is associated by dysregulated apoptosis of immune and non-immune cells. We investigated whether simvastatin has anti-apoptotic effects, and induces hepatocytes and lymphocytes survival signaling in endotoxin-induced liver and spleen injuries. Wistar rats were divided into the groups pretreated with simvastatin (20 or 40 mg/kg, orally) prior to a non-lethal dose of lipopolysaccharide (LPS), the LPS group, and the control. The severity of tissue inflammatory injuries was expressed as hepatic damage scores (HDS) and spleen damage scores (SDS), respectively. The apoptotic cell was detected by TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) and immunohistochemical staining (expression of cleaved caspase-3, and anti-apoptotic Bcl-xL, survivin and NF-kappa B/p65). Simvastatin dose-dependently abolished HDS and SDS induced by LPS (p &lt; 0.01), respectively. Simvastatin 40 mg/kg significantly decreased apoptotic index and caspase-3 cleavage in hepatocytes and lymphocytes (p &lt; 0.01 vs. LPS group, respectively), while Bcl-XL markedly increased accordingly with simvastatin doses. In the simvastatin, groups were determined markedly increased cytoplasmic expression of survivin associated with nuclear positivity of NF-kappa B, in both hepatocytes and lymphocytes (p &lt; 0.01 vs. LPS group). Cell-protective effects of simvastatin against LPS seemed to be mediated by up-regulation of survivin, which leads to reduced caspase-3 activation and inhibition of hepatocytes and lymphocytes apoptosis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FRONTIERS IN PHARMACOLOGY

ISSN

1663-9812

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

February

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

1-13

UT code for WoS article

000458784700001

EID of the result in the Scopus database

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