Delta-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F20%3A50016726" target="_blank" >RIV/62690094:18470/20:50016726 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1420-3049/25/2/259" target="_blank" >https://www.mdpi.com/1420-3049/25/2/259</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules25020259" target="_blank" >10.3390/molecules25020259</a>
Alternative languages
Result language
angličtina
Original language name
Delta-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach
Original language description
Owing to their high surface area, stability, and functional groups on the surface, iron oxide hydroxide nanoparticles have attracted attention as enzymatic support. In this work, a chemometric approach was performed, aiming at the optimization of the horseradish peroxidase (HRP) immobilization process on Delta-FeOOH nanoparticles (NPs). The enzyme/NPs ratio (X1), pH (X2), temperature (X3), and time (X4) were the independent variables analyzed, and immobilized enzyme activity was the response variable (Y). The effects of the factors were studied using a factorial design at two levels (-1 and 1). The biocatalyst obtained was evaluated for the ferulic acid (FA) removal, a pollutant model. The materials were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The SEM images indicated changes in material morphology. The independent variables X1 (-0.57), X2 (0.71), and X4 (0.42) presented the significance effects estimate. The variable combinations resulted in two significance effects estimates, X1*X2 (-0.57) and X2*X4 (0.39). The immobilized HRP by optimized conditions (X1 = 1/63 (enzyme/NPs ratio, X2 = pH 8, X4 = 60 degrees C, and 30 min) showed high efficiency for FA oxidation (82%).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecules
ISSN
1420-3049
e-ISSN
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Volume of the periodical
25
Issue of the periodical within the volume
2
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
"Article Number: 259"
UT code for WoS article
000515381800021
EID of the result in the Scopus database
2-s2.0-85082146739