Agroinfiltration mediated scalable transient gene expression in genome edited crop plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F21%3A50018425" target="_blank" >RIV/62690094:18470/21:50018425 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/21:10433608

Result on the web

<a href="https://www.mdpi.com/1422-0067/22/19/10882" target="_blank" >https://www.mdpi.com/1422-0067/22/19/10882</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms221910882" target="_blank" >10.3390/ijms221910882</a>

Result language

angličtina

Original language name

Agroinfiltration mediated scalable transient gene expression in genome edited crop plants

Original language description

Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providing a large picture through pilot-scale experiment via transient approach. The direct delivery of recombinant agrobacteria through this approach carrying CRISPR/Cas cassette to knockout the expression of the target gene in the intercellular tissue spaces by physical or vacuum infiltration can simplify the targeted site modification. This review aims to provide information on Agrobacterium-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. This will ease the screening of numerous functions of genes in different plant species with wider applicability in future. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10402 - Inorganic and nuclear chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Volume of the periodical

22

Issue of the periodical within the volume

19

Country of publishing house

CH - SWITZERLAND

Number of pages

35

Pages from-to

"Article number: 10882"

UT code for WoS article

000709839900001

EID of the result in the Scopus database

2-s2.0-85116533592