1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F25%3A50021701" target="_blank" >RIV/62690094:18470/25:50021701 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s00344-024-11481-x" target="_blank" >https://link.springer.com/article/10.1007/s00344-024-11481-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00344-024-11481-x" target="_blank" >10.1007/s00344-024-11481-x</a>

Result language

angličtina

Original language name

1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors

Original language description

Ethylene is a pivotal regulator of coffee anthesis, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, offers a potential to manage flowering in coffee plants. This study aimed to identify the effect of 1-MCP on gene expression of ethylene receptors, and compare the interaction in silico between 1-MCP and different ethylene receptors to understand what could be associated with coffee anthesis promotion by this molecule. The study involved phenotypic characterization, ethylene quantification, RNA expression profiling, phylogenetic analysis, homology modeling, and molecular docking. Results revealed that 1-MCP accelerated floral bud development to anthesis within 12 days, contrasting with water treated floral buds which exhibited progress just after a rainfall event. Moreover, 1-MCP application notably increased ethylene levels in both leaves and floral buds. Gene expression analysis indicated that 1-MCP modulates ethylene related gene expression differently in leaves and floral buds. Decreasing the expression of signaling genes in floral buds (CaERS1-like, ETR2-like, ETR4-like and EIN4-like), increasing it in leaves (ETR1-like, ETR2-like, ETR4-like and CaCTR1-like); and increasing the expression of CaACO3-like (biosynthesis related gene) in floral buds. Molecular docking studies suggested that ethylene may exhibit a higher affinity for receptors with lower interaction energy with 1-MCP. In conclusion, 1-MCP could be a promising tool to promote anthesis in coffee trees by modulating ethylene related processes, as the interaction between 1-MCP and ethylene receptors may influence ethylene sensitivity through different modulation of gene expression in leaves and floral buds.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of plant growth regulation

ISSN

0721-7595

e-ISSN

1435-8107

Volume of the periodical

44

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

731-747

UT code for WoS article

001312262300003

EID of the result in the Scopus database

2-s2.0-85204132793