Investigation Into the Impact of Solvents on the Phytochemical Composition, Antioxidant Capacities, and Antihyperglycemic Activities of Erigeron annuus (L.) Pers.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F25%3A50023103" target="_blank" >RIV/62690094:18470/25:50023103 - isvavai.cz</a>

Alternative codes found

RIV/61989100:27360/25:10258918

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1155/bmri/6650124" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1155/bmri/6650124</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/bmri/6650124" target="_blank" >10.1155/bmri/6650124</a>

Result language

angličtina

Original language name

Investigation Into the Impact of Solvents on the Phytochemical Composition, Antioxidant Capacities, and Antihyperglycemic Activities of Erigeron annuus (L.) Pers.

Original language description

This study aims to assess the phytochemical composition, antioxidant potential, and antidiabetic properties of Erigeron annuus (L.) Pers. The ethyl acetate fraction of Erigeron annuus leaves exhibited the highest extraction rate (22.42%). The preliminary qualitative phytochemical analysis in crude extract and fractions is often performed using chemical tests. For quantitative analysis, spectrophotometric methods are widely used to estimate the concentration of phytochemicals. The antioxidant properties were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric reducing antioxidant power (FRAP) assay, which measures the reduction of Fe3+ to Fe2+. Qualitative screening revealed the presence of tannins, flavonoids, phenols, saponins, and alkaloids. Notably, the ethyl acetate fraction showed significantly (p &lt; 0.05) higher total phenolic content (70.01 +/- 1.1 mg/g) and total flavonoid content (80.29 +/- 1.03 mg/g). This fraction also demonstrated substantial alpha-amylase inhibitory activity and antioxidant potential, suggesting the ability of polyphenols to reduce alpha-amylase activity. The alpha-amylase inhibition (23.15 +/- 1.22% to 67.31 +/- 2.01%) activity and IC50 value (40.59 +/- 0.03 mu g/mL) were notably higher in the ethyl acetate fraction compared with the standard drug metformin (19.88 +/- 1.51 mu g/mL). Erigeron annuus ethyl acetate fraction exhibited significantly higher glucose levels (10.88% +/- 1.29% to 65.11 +/- 0.94%) and conducted a lipid peroxidation experiment utilizing egg yolk as the source of lipids with high content. The most bioactive fraction was evaluated for cytotoxicity against the HEK293 cell line. The cytotoxicity assay revealed that 50% cell viability was observed at a concentration of 50 mu g/mL, indicating that the plant extract is nontoxic at concentrations below this threshold. Furthermore, the dominant fraction was further investigated using liquid chromatography-mass spectroscopy and high-performance thin-layer chromatography techniques from the selected plant. Moreover, an in vivo study will be performed to evaluate the antidiabetic efficacy of Erigeron annuus, isolate and characterize its bioactive components, and examine its molecular mechanism of action to improve its therapeutic applicability.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30108 - Toxicology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BioMed Research International

ISSN

2314-6133

e-ISSN

2314-6141

Volume of the periodical

2025

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

21

Pages from-to

"Article Number: 6650124"

UT code for WoS article

001467183800001

EID of the result in the Scopus database

2-s2.0-105003147958