Human embryonic stem cells are capable of executing G1/S checkpoint activation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F10%3A%230000915" target="_blank" >RIV/65269705:_____/10:#0000915 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/10:00350028 RIV/00216224:14110/10:00067228

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Human embryonic stem cells are capable of executing G1/S checkpoint activation

Original language description

Embryonic stem cells progress very rapidly through the cell cycle, allowing limited time for cell cycle regulatory circuits that typically function in somatic cells. Mechanisms that inhibit cell cycle progression upon DNA damage are of particular importance, as their malfunction may contribute to the genetic instability observed in human embryonic stem cells (hESCs). In this study, we exposed undifferentiated hESCs to DNA-damaging UVC light and examined their progression through the G1/S transition. Weshow that hESCs irradiated in G1 phase undergo cell cycle arrest before DNA synthesis and exhibit decreased cyclin-dependent kinase 2 (CDK2) activity. We also show that the phosphatase Cdc25A, which directly activates CDK2, is downregulated in irradiatedhESCs through the action of the checkpoint kinases Chk1 and/or Chk2. Importantly, the classical effector of the p53-mediated pathway, protein p21, is not a regulator of G1/S progression in hESCs.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/NS10439" target="_blank" >NS10439: Analysis of changes in genome and its expression in chronic lymphocytic leukemia patients during the disease development</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Stem cells

ISSN

1066-5099

e-ISSN

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Volume of the periodical

28

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

280746400004

EID of the result in the Scopus database

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