?Mycobacterium avium subsp. Hominissuis? in Neck Lymph Nodes of Children and their Enviroment Examined by Culture and Triplex Quantitative Real-Time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F11%3A%230001248" target="_blank" >RIV/65269705:_____/11:#0001248 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1128/JCM.00802-10" target="_blank" >http://dx.doi.org/10.1128/JCM.00802-10</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JCM.00802-10" target="_blank" >10.1128/JCM.00802-10</a>

Result language
angličtina
Original language name
?Mycobacterium avium subsp. Hominissuis? in Neck Lymph Nodes of Children and their Enviroment Examined by Culture and Triplex Quantitative Real-Time PCR
Original language description
Mycobacterium avium subsp. hominissuis? often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment. We confirmed the diagnoses by detecting M. avium subsp. hominissuis using qPCR with DNA directly isolated from lymph node biopsy specimens of two patients. In order to trace the source of infection from the environment, a method of DNA isolation from soil and other environmental samples, such as dust, cobwebs, andcompost, was developed. The triplex qPCR examination revealed the presence of M. avium subsp. hominissuis in a high proportion of the environmental samples (42.8% in the first patient?s house and 47.6% in the second patient?s house). Both
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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