Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genogypes are Related to the Sequence of the SS14 Strain

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F12%3A%230001785" target="_blank" >RIV/65269705:_____/12:#0001785 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2340/0001555-1335" target="_blank" >http://dx.doi.org/10.2340/0001555-1335</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2340/00015555-1335" target="_blank" >10.2340/00015555-1335</a>

Result language

angličtina

Original language name

Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genogypes are Related to the Sequence of the SS14 Strain

Original language description

A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA wasbased on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37,3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FE - Other fields of internal medicine

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Dermato-Venereologica

ISSN

0001-5555

e-ISSN

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Volume of the periodical

92

Issue of the periodical within the volume

6

Country of publishing house

SE - SWEDEN

Number of pages

6

Pages from-to

669-674

UT code for WoS article

000310828300030

EID of the result in the Scopus database

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