Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F14%3A00061623" target="_blank" >RIV/65269705:_____/14:00061623 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/14:00077729
Result on the web
<a href="http://dx.doi.org/10.1016/j.jcv.2014.07.008" target="_blank" >http://dx.doi.org/10.1016/j.jcv.2014.07.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jcv.2014.07.008" target="_blank" >10.1016/j.jcv.2014.07.008</a>

Result language
angličtina
Original language name
Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR
Original language description
Background: Antiviral resistance development is a serious complication of human cytomegalovirus virostatic therapy caused by mutations in UL 97 and/or UL54 genes. Objectives: To determinate the presence of sensitive and resistant strains in patients developing antiviral resistance. Study design: We used three different molecular biological methods for mutation analysis-restriction fragment length polymorphism, sequencing and real-time PCR approach. Results: We describe three allogeneic hematopoietic stem cell transplant patients developing the GCV resistant HCMV strains manifested by virostatic treatment failure. In these patients we identified UL97 mutations L595S, A594V and A594T and monitored the dynamics of coexisted sensitive/resistant strains. Weconfirmed the presence of mixed HCMV populations and in two patients a phenomenon of sensitive strain repopulation which occurred after 6.5 months and 1 month after removing GCV pressure. Conclusions: Our results show changes in proporti
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Project
<a href="/en/project/NT13691" target="_blank" >NT13691: Risk factors for development of CMV virostatic resistance in the patients after allogeneic stem cell transplantation.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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