Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F16%3A00065595" target="_blank" >RIV/65269705:_____/16:00065595 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/16:00091214
Result on the web
<a href="http://dx.doi.org/10.1017/S0950268816000972" target="_blank" >http://dx.doi.org/10.1017/S0950268816000972</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S0950268816000972" target="_blank" >10.1017/S0950268816000972</a>
Alternative languages
Result language
angličtina
Original language name
Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients
Original language description
To examine evidence of positive antibodies against immunogenic proteins of Anaplasma phagocytophilum in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific Anaplasma antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (n = 47) and from blood donors (n = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (20%) showed positive IgM reactions and seven samples (24%) were IgG positive for A. phagocytophilum by immunoblot. Borderline reactivity was found in 30 samples (1014%) for IgM and 36 samples (122%) for IgG. The difference between patients and blood donors was statistically significant for IgM (P = 0006) and for IgG (P = 00007) antibodies. A statistically significant result was obtained for IgG (P = 002) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to A. phagocytophilum in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
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Result continuities
Project
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Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Epidemiology and Infection
ISSN
0950-2688
e-ISSN
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Volume of the periodical
144
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
2597-2604
UT code for WoS article
000382174600015
EID of the result in the Scopus database
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