Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F17%3A00067300" target="_blank" >RIV/65269705:_____/17:00067300 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/17:00097722

Result on the web

<a href="https://ac.els-cdn.com/S1566070216302594/1-s2.0-S1566070216302594-main.pdf?_tid=913473ce-c88c-11e7-ae6b-00000aacb35e&acdnat=1510589352_7a9bb3a5d784b8dcfd4cc6a4653402a7" target="_blank" >https://ac.els-cdn.com/S1566070216302594/1-s2.0-S1566070216302594-main.pdf?_tid=913473ce-c88c-11e7-ae6b-00000aacb35e&acdnat=1510589352_7a9bb3a5d784b8dcfd4cc6a4653402a7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.autneu.2017.06.003" target="_blank" >10.1016/j.autneu.2017.06.003</a>

Result language

angličtina

Original language name

Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

Original language description

Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14 days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naive femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin +/SDF1 + mesenchymal stem cells and c-Kit +/CD90 + hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Autonomic Neuroscience-Basic &amp; Clinical

ISSN

1566-0702

e-ISSN

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Volume of the periodical

206

Issue of the periodical within the volume

SEP 2017

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

19-27

UT code for WoS article

000408178900003

EID of the result in the Scopus database

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