Epithelium morphogenesis and oviduct development are regulated by significant increase of expression of genes after long-term in vitro primary culture – a microarray assays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00069408" target="_blank" >RIV/65269705:_____/18:00069408 - isvavai.cz</a>
Result on the web
<a href="https://content.sciendo.com/view/journals/acb/6/4/article-p195.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/6/4/article-p195.xml</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/acb-2018-0030" target="_blank" >10.2478/acb-2018-0030</a>
Alternative languages
Result language
angličtina
Original language name
Epithelium morphogenesis and oviduct development are regulated by significant increase of expression of genes after long-term in vitro primary culture – a microarray assays
Original language description
The correct oviductal development and morphogenesis of its epithelium are crucial factors influencing female fertility. Oviduct is involved in maintaining an optimal environment for gametes and preimplantation embryo development; secretory oviductal epithelial cells (OECs) synthesize components of oviductal fluid. Oviductal epithelium also participates in sperm binding and its hyperactivation. For better understanding of the genetic bases that underlay porcine oviductal development, OECs were isolated from porcine oviducts and established long-term primary culture. A microarray approach was utilized to determine the differentially expressed genes during specific time periods. Cells were harvested on day 7, 15 and 30 of in vitro primary culture and their RNA was isolated. Gene expression was analyzed and statistical analysis was performed. 48 differentially expressed genes belonging to "tube morphogenesis", "tube development", "morphogenesis of an epithelium", "morphogenesis of branching structure" and "morphogenesis of branching epithelium" GO BP terms were selected, of which 10 most upregulated include BMP4, ARG1, SLIT2, FGFR1, DAB2, TNC, EPAS1, HHEX, ITGB3 and LOX. The results help to shed light on the porcine oviductal development and its epithelial morphogenesis, and show that after long-term culture the OECs still proliferate and maintain their tube forming properties.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Medical Journal of Cell Biology
ISSN
2544-3577
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
4
Country of publishing house
PL - POLAND
Number of pages
10
Pages from-to
"195–204"
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85060373994