A preliminary analysis of somatic exome mutations of minimal residual disease in multiple myeloma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00070060" target="_blank" >RIV/65269705:_____/18:00070060 - isvavai.cz</a>
Result on the web
<a href="https://www.prolekare.cz/casopisy/transfuze-hematologie-dnes/2018-supplementum-2/posterova-sekce-105545" target="_blank" >https://www.prolekare.cz/casopisy/transfuze-hematologie-dnes/2018-supplementum-2/posterova-sekce-105545</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
A preliminary analysis of somatic exome mutations of minimal residual disease in multiple myeloma
Original language description
Our aim is to explore mutational spectrum in minimal residual disease (MRD) of multiple myeloma (MM) with focus on possible treatment targets. Aberrant plasma cells were collected according to surface markers CD38, CD45, CD56 and CD19 from 7 MRD patients treated with Velclade based regimen. DNA was isolated and amplified by the Single cell amplification kit (QIAGEN). SureSelect Human All Exon V6 Kit was used to prepare libraries. 100 bp reads were sequenced on Illumina HiSeq 4000 platform with expected coverage 100x. Data were bioinformatically processed by BWA (hg38), VarScan2 and custom post-processing pipeline for annotation and filtering of variants. All resulting genes were compared with the M3P panel. We were able to detect genomic variants in 7 patients with MM MRD. This work represents a primer step towards the identification of new therapeutic targets in multiple myeloma minimal residual disease.
Czech name
—
Czech description
—
Classification
Type
O - Miscellaneous
CEP classification
—
OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/NV17-30089A" target="_blank" >NV17-30089A: In-depth genomic analysis of residual clone in multiple myeloma: approach for individualized targeted therapy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů