Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00070866" target="_blank" >RIV/65269705:_____/19:00070866 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/19:00113002
Result on the web
<a href="https://www.biolifesas.org/biolife/2019/02/19/insight-into-nuclear-cytoplasmic-shuttling-as-a-developmental-and-differentiational-capability-of-cells-in-primary-culture-models/" target="_blank" >https://www.biolifesas.org/biolife/2019/02/19/insight-into-nuclear-cytoplasmic-shuttling-as-a-developmental-and-differentiational-capability-of-cells-in-primary-culture-models/</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells
Original language description
The ovarian granulosa cells (GCs) that form the structure of follicle undergo substantial modification during the various stages of human folliculogenesis. These modifications include morphological changes, accompanied by differential expression of genes, encoding proteins which are mainly involved in cell growth, proliferation and differentiation. Recent data bring a new insight into the aspects of GCs' stem-like specificity and plasticity, enabling their prolonged proliferation and differentiation into other cell types. This manuscript focuses attention on emerging alterations during GC cell cycle - a series of biochemical and biophysical changes within the cell. Human GCs were collected from follicles of women set to undergo intracytoplasmic sperm injection procedure, as a part of remnant follicular fluid. The cells were primarily cultured for 30 days. Throughout this time, we observed the prominent change in cell morphology from epithelial-like to fibroblast-like, suggesting differentiation to other cell types. Additionally, at days 1, 7, 15 and 30, the RNA was isolated for molecular assays. Using Affymetrie (R) Human Genome U219 Array, we found 2579 human transcripts that were differentially expressed in GCs. From these genes, we extracted 582 Gene Ontology Biological Process (GO BP) Terms and 45 KEGG pathways, among which we investigated transcripts belonging to four GO BPs associated ith cell proliferation: "cell cycle phase transition", "Gl/S phase transition", G2/M phase transition" and "cell cycle checkpoint". Microarray results were validated by RT-qPCR. Increased expression of all the genes studied indicated that increase in GC proliferation during long-term in vitro culture is orchestrated by the up-regulation of genes related to cell cycle control. Furthermore, observed changes in cell morphology may be regulated by a presented set of genes, leading to the induction of pathways specific for sternness plasticity and transdifferentiation in vitro.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Regulators and Homeostatic Agents
ISSN
0393-974X
e-ISSN
—
Volume of the periodical
33
Issue of the periodical within the volume
1
Country of publishing house
IT - ITALY
Number of pages
13
Pages from-to
39-51
UT code for WoS article
000464697500005
EID of the result in the Scopus database
—