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Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00070866" target="_blank" >RIV/65269705:_____/19:00070866 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/19:00113002

  • Result on the web

    <a href="https://www.biolifesas.org/biolife/2019/02/19/insight-into-nuclear-cytoplasmic-shuttling-as-a-developmental-and-differentiational-capability-of-cells-in-primary-culture-models/" target="_blank" >https://www.biolifesas.org/biolife/2019/02/19/insight-into-nuclear-cytoplasmic-shuttling-as-a-developmental-and-differentiational-capability-of-cells-in-primary-culture-models/</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells

  • Original language description

    The ovarian granulosa cells (GCs) that form the structure of follicle undergo substantial modification during the various stages of human folliculogenesis. These modifications include morphological changes, accompanied by differential expression of genes, encoding proteins which are mainly involved in cell growth, proliferation and differentiation. Recent data bring a new insight into the aspects of GCs&apos; stem-like specificity and plasticity, enabling their prolonged proliferation and differentiation into other cell types. This manuscript focuses attention on emerging alterations during GC cell cycle - a series of biochemical and biophysical changes within the cell. Human GCs were collected from follicles of women set to undergo intracytoplasmic sperm injection procedure, as a part of remnant follicular fluid. The cells were primarily cultured for 30 days. Throughout this time, we observed the prominent change in cell morphology from epithelial-like to fibroblast-like, suggesting differentiation to other cell types. Additionally, at days 1, 7, 15 and 30, the RNA was isolated for molecular assays. Using Affymetrie (R) Human Genome U219 Array, we found 2579 human transcripts that were differentially expressed in GCs. From these genes, we extracted 582 Gene Ontology Biological Process (GO BP) Terms and 45 KEGG pathways, among which we investigated transcripts belonging to four GO BPs associated ith cell proliferation: &quot;cell cycle phase transition&quot;, &quot;Gl/S phase transition&quot;, G2/M phase transition&quot; and &quot;cell cycle checkpoint&quot;. Microarray results were validated by RT-qPCR. Increased expression of all the genes studied indicated that increase in GC proliferation during long-term in vitro culture is orchestrated by the up-regulation of genes related to cell cycle control. Furthermore, observed changes in cell morphology may be regulated by a presented set of genes, leading to the induction of pathways specific for sternness plasticity and transdifferentiation in vitro.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30105 - Physiology (including cytology)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biological Regulators and Homeostatic Agents

  • ISSN

    0393-974X

  • e-ISSN

  • Volume of the periodical

    33

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    IT - ITALY

  • Number of pages

    13

  • Pages from-to

    39-51

  • UT code for WoS article

    000464697500005

  • EID of the result in the Scopus database