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Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3D culture system

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00071862" target="_blank" >RIV/65269705:_____/19:00071862 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/19:00113277

  • Result on the web

    <a href="http://cost-cellfit.eu/wp-content/uploads/2019/10/Proceedings-Annual-Meeting2019.pdf" target="_blank" >http://cost-cellfit.eu/wp-content/uploads/2019/10/Proceedings-Annual-Meeting2019.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Anti-apoptotic and pro-apoptotic factors in human granulosa cells cultured in 3D culture system

  • Original language description

    Granulosa cells have several functions during follicular development; for example, theynurse the oocyte, regulate steroid hormones production and activity, and prevent oocyte against apoptosis. For these abilities, granulosa cells are crucial for many reproductive functions and are suitable for in vitro culture and testing. However, when granulosa cells are cultured in vitro as monolayers, they gradually lose most of their important functions. The aim of our study was to test the three dimensional (3D) culture system for human granulosa cells. For this purpose nanofibrous cellulose hydrogel GrowDex was used. The expression of pro-and anti-apoptotic factors in human granulosa cells was investigated with the target to verify the usability of the 3D system with GrowDex. Human granulosa cells denuded from oocytes were cultured for 16 days in 4-well NUNC culture dishes with GrowDex. Conventional 2D (monolayer) culture system was used asa control. After prolong 16 days culture the expression of pro-and anti-apoptoticmarkers; Bax and BCl 2 respectively, was examined by Western blotting. The cells cultured in 3D culture system expressed anti-apoptotic factor BCl 2 in higher amounts than cells cultured in the conventional 2D culture system. Besides, the expression ofpro-apoptotic factor Bax was lower in the 3D system than the expression in the conventional culture system.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    <a href="/en/project/LTC18059" target="_blank" >LTC18059: Cultivation system suitable for long-term cultivation of human follicular cells</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů