Differential expression pattern of genes involved in oxygen metabolism in epithelial oviductal cells during primary in vitro culture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00072384" target="_blank" >RIV/65269705:_____/19:00072384 - isvavai.cz</a>

Result on the web

<a href="https://content.sciendo.com/view/journals/acb/7/2/article-p66.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/7/2/article-p66.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2019-0009" target="_blank" >10.2478/acb-2019-0009</a>

Result language

angličtina

Original language name

Differential expression pattern of genes involved in oxygen metabolism in epithelial oviductal cells during primary in vitro culture

Original language description

Oxygen metabolism is crucial in establishing successful pregnancy, since excessive amount of reactive oxygen species (ROS) may exert deleterious effects on the developing embryo. There are several defense mechanisms against oxidative stress in the female reproductive tract, including production of antioxidant enzymes by oviductal epithelial cells (OECs). Undoubtedly, OECs play major part in female fertility and may also serve as an in vitro model of the oviduct. Therefore, the aim of this study was to investigate the expression of genes involved in oxygen metabolism. We have isolated OECs from oviducts of crossbred gilts (n=45) and maintained their in vitro culture for 30 days, collecting their RNA at days 1, 7, 15 and 30. The gene expression was determined with the use of Affymetrix(R) Porcine Gene 1.1 ST Array Strip. Our results revealed 166 differentially expressed genes belonging to four ontology groups: .,cellular response to oxidative stress&quot;, &quot;cellular response to oxygen-containing compound&quot;, &quot;cellular response to oxygen levels&quot; and &quot;cellular response to reactive oxygen species&quot;, most of which are also involved in other major processes in the organism. However, our findings provide a valuable insight into porcine reproductive biology and may be utilized in optimization of assisted reproduction techniques. Running title: Genes involved in oxygen metabolism in oviductal epithelial cells

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

2

Country of publishing house

PL - POLAND

Number of pages

11

Pages from-to

66-76

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85074585697