Light sheet fluorescence microscopy of optically cleared brains for studying the glymphatic system

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F20%3A00073967" target="_blank" >RIV/65269705:_____/20:00073967 - isvavai.cz</a>
Result on the web
<a href="https://journals.sagepub.com/doi/abs/10.1177/0271678X20924954?journalCode=jcba" target="_blank" >https://journals.sagepub.com/doi/abs/10.1177/0271678X20924954?journalCode=jcba</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/0271678X20924954" target="_blank" >10.1177/0271678X20924954</a>

Result language
angličtina
Original language name
Light sheet fluorescence microscopy of optically cleared brains for studying the glymphatic system
Original language description
Fluid transport in the perivascular space by the glia-lymphatic (glymphatic) system is important for the removal of solutes from the brain parenchyma, including peptides such as amyloid-beta which are implicated in the pathogenesis of Alzheimer's disease. The glymphatic system is highly active in the sleep state and under the influence of certain of anaesthetics, while it is suppressed in the awake state and by other anaesthetics. Here we investigated whether light sheet fluorescence microscopy of whole optically cleared murine brains was capable of detecting glymphatic differences in sleep- and awake-mimicking anaesthesia, respectively. Using light-sheet imaging of whole brains, we found anaesthetic-dependent cerebrospinal fluid (CSF) influx differences, including reduced tracer influx along tertiary branches of the middle cerebral artery and reduced influx along dorsal and anterior penetrating arterioles, in the awake-mimicking anaesthesia. This study establishes that light sheet microscopy of optically cleared brains is feasible for quantitative analyses and can provide images of the entire glymphatic system in whole brains.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30103 - Neurosciences (including psychophysiology)

Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)