Elevated epidermal growth factor receptor levels in Barett´s esophagus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F21%3A00074795" target="_blank" >RIV/65269705:_____/21:00074795 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/ueg2.12144" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/ueg2.12144</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ueg2.12144" target="_blank" >10.1002/ueg2.12144</a>
Alternative languages
Result language
angličtina
Original language name
Elevated epidermal growth factor receptor levels in Barett´s esophagus
Original language description
Introduction Gastroesophageal reflux disease (GERD) is a chronic gastrointestinal illness characterized by the reflux of gastric contents causing symptoms and/or complications such as reflux esophagitis (RE) or Barrett's esophagus (BE), the precursor of esophageal adenocarcinoma (EAC). The epidermal growth factor (EGF) and its receptor (EGFR) is pivotal in the maintenance of the integrity of the esophageal mucosa and repair of injured tissues. On the other hand, upregulation of the EGF signaling pathway has been shown to lead to neoplastic transformation; in addition, EGFR expression was also associated with GERD progression.1-5 Aims & Methods The aim of our study was to compare the expression of EGF and EGFR in the esophageal tissues with/without pathological changes in GERD patients. The EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in 50 fresh esophageal tissue biopsies with/without pathological changes taken from 25 patients with complications of GERD (RE [N=10], BE [N=8], EAC [N=7]). In addition, the EGFR protein expression was examined in 50 corresponding formalin-fixed paraffin esophageal tissues using immunohistochemistry. The mRNA and protein expressions were statistically evaluated using the Wilcoxon signed ranked test in Statistica v13.2. Results No differences in the EGF or EGFR mRNA expressions in the esophageal tissues with/without pathological changes were detected in neither of the patient groups (p>0.05). However, the EGFR protein levels in BE biopsies were higher than in corresponding samples without metaplastic changes (p=0.034). Conclusion Although the study is limited by the small number of patients, our results suggest that EGFR may possibly serve as a biomarker of BE development. This is the first study comparing EGF and its receptor expressions in multiple sites of the esophagus in the same patient with GERD.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30200 - Clinical medicine
Result continuities
Project
<a href="/en/project/NU20-03-00126" target="_blank" >NU20-03-00126: Host microbiome in relation to Barrett´s esophagus and esophageal adenocarcinoma development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů