Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F22%3A00077793" target="_blank" >RIV/65269705:_____/22:00077793 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/22:00128888
Result on the web
<a href="https://journals.muni.cz/CPR/article/view/33535/28610" target="_blank" >https://journals.muni.cz/CPR/article/view/33535/28610</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5817/CPR2022-2-20" target="_blank" >10.5817/CPR2022-2-20</a>
Alternative languages
Result language
angličtina
Original language name
Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
Original language description
In our study, we focused on the growth of three different microautotrophs isolated from Antarctic lichens (Placopsis contortuplicata, Solorina spongiosa) and cryptoendolithic algal vegetation. The isolates were purified and inoculated on agar plates, Bold ' s Basal Medium (BBM). The growth of the cultures and the markers of physiological (photo-synthetic) activity were monitored by chlorophyll fluorescence in 1 week intervals for 3 months after inoculation. For the assessment of photosynthetic activity, the meth-od of slow Kautsky kinetics supplemented with saturation pulses was applied. Four chlorophyll fluorescence parameters calculated: (1) maximum quantum yield of PSII (FV/FM), (2) effective quantum yield of photosynthetic processes in PSII (phi PSII), (3) non -photochemical quenching of chlorophyll fluorescence, and (4) background chlorophyll fluorescence ratio (F0/F0 '). Troughout the cultivation period, the maximum quantum yield of PSII (FV/FM) showed high values in all three autotrophs with only slight increase in the first part of the cultivation period, followed by slight decrease in the second part. The phi PSII values showed a rapid decline within the first 4 weeks of cultivation followed by more or less constant values in the isolates from P. contortuplicata and cryptoen-dolithic alga. Contrastingly, time course of phi PSII rather showed an increase followed by a decrease in S. spongiosa isolate. NPQ (related to the activation of protective mecha-nisms) increased in the second part of cultivation period, the rate of increase and maxi-mum values were species-specific. The species-specific differences in chlorophyll fluo-rescence parameters are discussed as well as their potential for evaluation of photo-synthetic performance of in vitro cultivated algal/cyanobacterial cultures on agar plates.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/EF16_013%2F0001708" target="_blank" >EF16_013/0001708: ECOPOLARIS - Changes in structure and function of components of terrestrial polar ecosystems (CzechPolar2)</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Polar Reports
ISSN
1805-0689
e-ISSN
1805-0697
Volume of the periodical
12
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
11
Pages from-to
269-279
UT code for WoS article
000935360100010
EID of the result in the Scopus database
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