New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F24%3A00079867" target="_blank" >RIV/65269705:_____/24:00079867 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/24:00136135
Result on the web
<a href="https://www.biolifesas.org/EN/10.23812/j.biol.regul.homeost.agents.20243804.219" target="_blank" >https://www.biolifesas.org/EN/10.23812/j.biol.regul.homeost.agents.20243804.219</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20243804.219" target="_blank" >10.23812/j.biol.regul.homeost.agents.20243804.219</a>
Alternative languages
Result language
angličtina
Original language name
New Gene Markers Regulating the Process of Vascularization in Pig Preovulatory Oocytes during Growth and Maturation
Original language description
Background: A frequent reason for in vitro fertilization failure is insufficient oocyte competence and difficulties in mimicking optimal in vivo conditions at the stage of in vitro maturation. This study aimed to identify the expression profile of genes associated with "vasculature development", "circulatory system process", "hemopoiesis", "patterning of blood vessels" and "hemopoietic or lymphoid organ development" ontological groups during in vitro maturation of domestic pig oocytes. The goal was also to describe new genetic markers that can be used in assisted reproduction techniques. Methods: Porcine oocytes were subjected to in vitro maturation, and the expression of selected genes was compared before and after maturation. Microarray and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis were performed on total RNA isolated from the samples. Additionally, immunocytochemical staining was performed and the histological morphology of ovaries was examined. Results: Regardless of the individual processes, the gene expression pattern remained the same and all 32 gene ontology biological process term-genes were represented in down-regulated gene sets. The greatest change in expression was shown by the Vascular Endothelial Growth Factor A (VEGFA) and the smallest by myoferlin (MYOF). Moreover, the expression of VEGFA was confirmed by immunohistochemical analysis of ovaries. Conclusions: The potential contribution of chosen genes to oocyte performance during in vitro maturation can enhance comprehension of the underlying processes in oocytes and their microenvironment. It may also improve the effectiveness of in vitro fertilization.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Regulators and Homeostatic Agents
ISSN
0393-974X
e-ISSN
1724-6083
Volume of the periodical
38
Issue of the periodical within the volume
4
Country of publishing house
IT - ITALY
Number of pages
18
Pages from-to
2807-2824
UT code for WoS article
001203519300001
EID of the result in the Scopus database
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