Endocrine disruption of adipose physiology: Screening in SGBS cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F24%3A00080288" target="_blank" >RIV/65269705:_____/24:00080288 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/24:00137604

Result on the web

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jat.4679" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jat.4679</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jat.4679" target="_blank" >10.1002/jat.4679</a>

Result language

angličtina

Original language name

Endocrine disruption of adipose physiology: Screening in SGBS cells

Original language description

The increasing use of industrial chemicals has raised concerns regarding exposure to endocrine-disrupting chemicals (EDCs), which interfere with developmental, reproductive and metabolic processes. Of particular concern is their interaction with adipose tissue, a vital component of the endocrine system regulating metabolic and hormonal functions. The SGBS (Simpson Golabi Behmel Syndrome) cell line, a well-established human-relevant model for adipocyte research, closely mimics native adipocytes&apos; properties. It responds to hormonal stimuli, undergoes adipogenesis and has been successfully used to study the impact of EDCs on adipose biology. In this study, we screened human exposure-relevant doses of various EDCs on the SGBS cell line to investigate their effects on viability, lipid accumulation and adipogenesis-related protein expression. Submicromolar doses were generally well tolerated; however, at higher doses, EDCs compromised cell viability, with cadmium chloride (CdCl2) showing the most pronounced effects. Intracellular lipid levels remained unaffected by EDCs, except for tributyltin (TBT), used as a positive control, which induced a significant increase. Analysis of adipogenesis-related protein expression revealed several effects, including downregulation of fatty acid-binding protein 4 (FABP4) by dibutyl phthalate, upregulation by CdCl2 and downregulation of perilipin 1 and FABP4 by perfluorooctanoic acid. Additionally, TBT induced dose-dependent upregulation of C/EBP alpha, perilipin 1 and FABP4 protein expression. These findings underscore the importance of employing appropriate models to study EDC-adipocyte interactions. Conclusions from this research could guide strategies to reduce the negative impacts of EDC exposure on adipose tissue. The increasing use of chemicals raises concerns about exposure to endocrine-disrupting chemicals (EDCs). This study used the Simpson Golabi Behmel Syndrome cell line, a human-relevant adipocyte model, to screen the effect of various EDCs on viability, lipid accumulation and adipogenesis-related protein expression. Submicromolar doses were generally well tolerated, and EDCs did not affect lipid levels except for tributyltin, which increased them and also upregulated FABP4, perilipin 1 and C/EBP alpha.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30108 - Toxicology

Project

<a href="/en/project/EF17_043%2F0009632" target="_blank" >EF17_043/0009632: CETOCOEN Excellence</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Toxicology

ISSN

0260-437X

e-ISSN

1099-1263

Volume of the periodical

44

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1784-1792

UT code for WoS article

001274671400001

EID of the result in the Scopus database

2-s2.0-85199411517