Bacteriome of the Middle Ear in Children and Young Adults With Cholesteatoma and Retraction Pocket: A Pilot Study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F25%3A00081603" target="_blank" >RIV/65269705:_____/25:00081603 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/25:00140801

Result on the web

<a href="https://aao-hnsfjournals.onlinelibrary.wiley.com/doi/full/10.1002/oto2.70051" target="_blank" >https://aao-hnsfjournals.onlinelibrary.wiley.com/doi/full/10.1002/oto2.70051</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/oto2.70051" target="_blank" >10.1002/oto2.70051</a>

Result language

angličtina

Original language name

Bacteriome of the Middle Ear in Children and Young Adults With Cholesteatoma and Retraction Pocket: A Pilot Study

Original language description

ObjectiveChronic otitis media (COM) is a common middle ear disease in children and young adults. Dysfunction of the Eustachian tube and bacterial infection are the main causes. This pilot study aimed to describe and compare bacteriomes of the middle ear in children and young adults with serious forms of COM, such as cholesteatoma and retraction pocket (RP) of the tympanic membrane, with bacteriomes in healthy middle ears.Study DesignObservational study.SettingClinical practice in a tertiary center. From January 1, 2021 to August 31, 2022. Patients aged 0 to 20 years.MethodsIn this case-control study, middle ears were swabbed during surgery on children with cholesteatoma (N = 23) or RP (N = 26) and on children indicated for cochlear implant (N = 15, controls). Genomic DNA extraction was followed by creation of a 16S ribosomal DNA gene library and sequencing on a MiSeq instrument. Samples with relative abundance of at least one bacterial genus &gt;20% were considered positive for the specific genus.ResultsBacterial diversity was generally low in the middle ear samples from patients with COM, with DNA content from 1 or 2 bacteria usually dominating in the sample. A significant difference in positivity for one or more bacterial genera was observed between patients with cholesteatoma or RP (38.8%) versus patients indicated for cochlear implants (6.7%).ConclusionWhile middle ear bacteriomes in cases of cholesteatoma and RP differed from those of controls, findings in the 2 pathological conditions were similar. These results support the statement that the RP could be a precholesteatoma stage.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30206 - Otorhinolaryngology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

OTO Open

ISSN

2473-974X

e-ISSN

2473-974X

Volume of the periodical

9

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

"e70051"

UT code for WoS article

001421283300001

EID of the result in the Scopus database

2-s2.0-85218929900