Bacterial genera in the fluids from apical periodontitis-related radicular cysts: An observational study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F25%3A00081757" target="_blank" >RIV/65269705:_____/25:00081757 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/25:00140928

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/iej.14220" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/iej.14220</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/iej.14220" target="_blank" >10.1111/iej.14220</a>

Result language

angličtina

Original language name

Bacterial genera in the fluids from apical periodontitis-related radicular cysts: An observational study

Original language description

Aim: This study aimed to evaluate bacteriome profiles (diversity, composition and relative abundances of bacterial genera) of the fluids from apical periodontitis (AP)-related radicular cysts (RCs). Methodology: This observational study included 29 patients with AP and RC with complete sample triplets (supragingival plaque, cryopulverized tooth and cystic fluid). The bacteriome profiles of each matrix as well as of negative controls (NCs) were examined using 16S rRNA amplicon sequencing. Results: Bacteriome profiles of cystic fluids differed from NCs in 79% of cases. The number of distinct amplicon sequence variants and Shannon index detected in cystic fluids and cryopulverized teeth were significantly lower than in paired supragingival plaque samples. Gram-negative genera and anaerobic genera were more abundant in cystic fluids than in paired cryopulverized teeth or their supragingival plaques. The relative abundances of the genera Prevotella_7/Prevotella, Fusobacterium and Porphyromonas were higher in cystic fluids than in paired cryopulverized teeth and NCs; their relative abundances dominated (&gt;20%) in individual cystic fluids. Also, DNA from the genus Fretibacterium was significantly more commonly found in cryopulverized teeth and cystic fluids than in supragingival plaque samples. The relative abundances of this gram-negative bacterial genera in cryopulverized teeth differed from NCs; the difference from cystic fluids was borderline insignificant. Conclusions: Although the alpha-diversity in the cystic fluids is much lower compared to supragingival plaques, most cystic fluids are not sterile. DNA from specific anaerobic gram-negative bacterial genera dominated the fluids from AP-related RCs.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30208 - Dentistry, oral surgery and medicine

Project

<a href="/en/project/NU20-08-00205" target="_blank" >NU20-08-00205: Molecular etiopathogenesis of apical periodontitis and odontogenic cystic lesions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Endodontic Journal

ISSN

0143-2885

e-ISSN

1365-2591

Volume of the periodical

58

Issue of the periodical within the volume

6

Country of publishing house

DK - DENMARK

Number of pages

14

Pages from-to

902-915

UT code for WoS article

001440024400001

EID of the result in the Scopus database

2-s2.0-105000131526