Resistance to ceftriaxone and penicillin G among contemporary syphilis strains confirmed by natural in vitro mutagenesis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F25%3A00082154" target="_blank" >RIV/65269705:_____/25:00082154 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/25:00141413
Result on the web
<a href="https://www.nature.com/articles/s43856-025-00948-x" target="_blank" >https://www.nature.com/articles/s43856-025-00948-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s43856-025-00948-x" target="_blank" >10.1038/s43856-025-00948-x</a>

Result language
angličtina
Original language name
Resistance to ceftriaxone and penicillin G among contemporary syphilis strains confirmed by natural in vitro mutagenesis
Original language description
BackgroundFor many years, syphilis treatment was considered straightforward due to the universal susceptibility of Treponema pallidum subsp. pallidum (TPA) to penicillin antibiotics.MethodsPenicillin-binding protein genes from a ceftriaxone treatment failure T. pallidum isolate were assessed, and the introduction of identified mutations into two laboratory strains via natural competence was aimed for, followed by in vitro analysis of antibiotic susceptibility of the recombinants.ResultsTPA from the ceftriaxone treatment failure case contained A1873G and G2122A mutations in the TP0705 gene. Introduction of the A1873G mutation into laboratory strains DAL-1 and SS14 resulted in partial resistance to ceftriaxone and penicillin G in vitro. Furthermore, in silico analyses revealed that the majority of contemporary TPA SS14-like strains harbors this mutation and are thus partially resistant to ceftriaxone and penicillin G.ConclusionsThis finding indicates that TPA strains accumulate mutations that increase their resistance to beta-lactam antibiotics. Alternative approaches for controlling syphilis will be needed, including the development of the syphilis vaccine.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30100 - Basic medicine

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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